Video link:
https://drive.google.com/uc?export=download&id=1oOfW4rbLgLDjZbqnzKQ6xDyIAiEnvOeuNote1 : The cells were transfected with 50nM of the target siRNA, 24h prior to live cell imaging (n= 229 cells, from 2 independent experiments).
Note2 : 38% of cells showed chromosome congression defects
Note3 : 4% of the cells died in mitosis without completing chromosome alignment; 6% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 191±276 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).