Kif18a_oligo2
Video link: https://drive.google.com/uc?export=download&id=1FBSpzERt9bx5_jaoDghUI_a20QolcsaB
Note1 : The cells were transfected with 50nM of the target siRNA, 24h prior to live cell imaging (n= 236 cells, from 2 independent experiments).
Note2 : 30% of cells showed chromosome congression defects
Note3 : 5% of the cells died in mitosis without completing chromosome alignment; 2% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 99±219 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).
Note1 : The cells were transfected with 50nM of the target siRNA, 24h prior to live cell imaging (n= 236 cells, from 2 independent experiments).
Note2 : 30% of cells showed chromosome congression defects
Note3 : 5% of the cells died in mitosis without completing chromosome alignment; 2% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 99±219 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).
Protein lysates obtained after siRNA were immunoblotted with an antibody specific to each protein of interest. Alfa-tubulin was used as loading control.