Video link:
https://drive.google.com/uc?export=download&id=17JJZqMAvh-pfeb3HfYbzQF-q984KxRkZNote1 : The cells were transfected with 50nM of the target siRNA, 48h prior to live cell imaging (n=428 cells, from 3 independent experiments).
Note2 : 23% of cells showed chromosome congression defects
Note3 : 1,4% of the cells died in mitosis without completing chromosome alignment;100% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 76±100 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).