SHP2_Oligo1
Video link: https://drive.google.com/uc?export=download&id=1fbmEWWSogmh4dV8rPFYulX0ueptl2x3S
Note1 : The cells were transfected with 50nM of the target siRNA, 48h prior to live cell imaging (n=347 cells, from 2 independent experiments).
Note2 : 76% of cells showed chromosome congression defects
Note3 : 9,2% of the cells died in mitosis without completing chromosome alignment; 4,3% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 144±103 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).
Note1 : The cells were transfected with 50nM of the target siRNA, 48h prior to live cell imaging (n=347 cells, from 2 independent experiments).
Note2 : 76% of cells showed chromosome congression defects
Note3 : 9,2% of the cells died in mitosis without completing chromosome alignment; 4,3% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 144±103 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).
Protein lysates obtained after siRNA were immunoblotted with an antibody specific to each protein of interest. GAPDH was used as loading control.