Video link:
https://drive.google.com/uc?export=download&id=1BW4NWlXDKd4pykPisIQUX5L_Tjhe5ceaNote1 : The cells were transfected with 50nM of the target siRNA, 24h prior to live cell imaging (n=560 cells, from 3 independent experiments).
Note2 : 16% of cells showed chromosome congression defects
Note3 : 1,1% of the cells died in mitosis without completing chromosome alignment; 4,8% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 71±101 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).