Video link:
https://drive.google.com/uc?export=download&id=1GWoozEY0n9iE1-FY-12ZwKfOw4sZ_tdVNote1 : The cells were transfected with 50nM of the target siRNA, 96h prior to live cell imaging (n=500 cells, from 3 independent experiments).
Note2 : 16% of cells showed chromosome congression defects
Note3 : 0% of the cells died in mitosis without completing chromosome alignment; 0,4% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 70±106 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).