Video link:
https://drive.google.com/uc?export=download&id=1ZfPTPZDhvDC58br7qdP5HgIEysCkgdmENote1 : The cells were transfected with 50nM of the target siRNA, 48h prior to live cell imaging (n=396 cells, from 2 independent experiments).
Note2 : 56% of cells showed chromosome congression defects
Note3 : 3,3% of the cells died in mitosis without completing chromosome alignment; 8,1% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 167±164 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).