Video link:
https://drive.google.com/uc?export=download&id=1bm64w4ANSn0rnmeVrGW3XlmcenYQkCn1Note1 : The cells were transfected with 50nM of the target siRNA, 48h prior to live cell imaging (n=462 cells, from 2 independent experiments).
Note2 : 24% of cells showed chromosome congression defects
Note3 : 5,2% of the cells died in mitosis without completing chromosome alignment; 3,3% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 92±174 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).