Video link:
https://drive.google.com/uc?export=download&id=18Mjfvmp6neDw7MtBdmDIm6KwNMKzZZugNote1 : The cells were transfected with 50nM of the target siRNA, 96h prior to live cell imaging (n=339 cells, from 3 independent experiments).
Note2 : 3% of cells showed chromosome congression defects
Note3 : 8,3% of the cells died in mitosis without completing chromosome alignment; 30,7% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 44±35 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).