Video link:
https://drive.google.com/uc?export=download&id=197nbUBOhRIwUdFe9sjXna73cuV_rhNOuNote1 : The cells were transfected with 50nM of the target siRNA, 48h prior to live cell imaging (n=329 cells, from 3 independent experiments).
Note2 : 50% of cells showed chromosome congression defects
Note3 : 4,3% of the cells died in mitosis without completing chromosome alignment; 17,6% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 130±194 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).