SPICE

Video link: https://drive.google.com/uc?export=download&id=10T0fnTBllx8p5vnMm5JIDvJsxgMfzIpj

Note1 : The cells were transfected with 50nM of the target siRNA, 48h prior to live cell imaging (n=504 cells, from 2 independent experiments).

Note2 : 14% of cells showed chromosome congression defects

Note3 : 0,8% of the cells died in mitosis without completing chromosome alignment; 2% of the cells died in the subsequent interphase

Note4 : The duration of mitosis was 79±158 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).