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Note1 : The cells were transfected with 50nM of the target siRNA, 96h prior to live cell imaging (n=400 cells, from 2 independent experiments).
Note2 : 4% of cells showed chromosome congression defects
Note3 : 0,5% of the cells died in mitosis without completing chromosome alignment; 0% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 40±23 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).