Video link:
https://drive.google.com/uc?export=download&id=1SX5PjFTXsZtmiyyKFS_BOxbdpmq7z5GANote1 : The cells were transfected with 50nM of the target siRNA, 96h prior to live cell imaging (n=355 cells, from 2 independent experiments).
Note2 : 8% of cells showed chromosome congression defects
Note3 : 1,4% of the cells died in mitosis without completing chromosome alignment; 0,9% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 55±105 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).