Video link:
https://drive.google.com/uc?export=download&id=1L8VP2lUJfT4lso9I2qyz7hRWNOz3bF6DNote1 : The cells were transfected with 50nM of the target siRNA, 48h prior to live cell imaging (n=377 cells, from 2 independent experiments).
Note2 : 37% of cells showed chromosome congression defects
Note3 : 4,2% of the cells died in mitosis without completing chromosome alignment; 4,2% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 146±239min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).