Video link:
https://drive.google.com/uc?export=download&id=1TRFrm3j4V6qBh55-eHeAEO1fvH-40-86Note1 : The cells were transfected with 50nM of the target siRNA, 72h prior to live cell imaging (n=566 cells, from 3 independent experiments).
Note2 : 49% of cells showed chromosome congression defects
Note3 : 14,3% of the cells died in mitosis without completing chromosome alignment; 17% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 174±252 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).