Video link:
https://drive.google.com/uc?export=download&id=1b95valqfO7cljned3aXF_66dZXLceLQANote1 : The cells were transfected with 50nM of the target siRNA, 72h prior to live cell imaging (n=399 cells, from 2 independent experiments).
Note2 : 15% of cells showed chromosome congression defects
Note3 : 0,5% of the cells died in mitosis without completing chromosome alignment; 1% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 72±117 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).