Video link:
https://drive.google.com/uc?export=download&id=1FPLJcTSA5J-raTmvTdx6RFiF0G1J0FLoNote1 : The cells were transfected with 50nM of the target siRNA, 48h prior to live cell imaging (n=338 cells, from 2 independent experiments).
Note2 : 24% of cells showed chromosome congression defects
Note3 : 1,5% of the cells died in mitosis without completing chromosome alignment; 4,1% of the cells died in the subsequent interphase
Note4 : The duration of mitosis was 90±161 min (mean±sd, determined by measuring the time between nuclear envelope breakdown (NEBD) to anaphase onset (AO)).